Selected article for: "chain reaction and PCR cycle"
Author: Bjarnason, Agnar; Westin, Johan; Lindh, Magnus; Andersson, Lars-Magnus; Kristinsson, Karl G; Löve, Arthur; Baldursson, Olafur; Gottfredsson, Magnus
Title: Incidence, Etiology, and Outcomes of Community-Acquired Pneumonia: A Population-Based Study
  • Document date: 2018_2_8
    • ID: sw8ghj6q_20
    Snippet: Diarrhea 49 (16) 12 (20) 9 (25) 3 (14) 1 (13) with rhinovirus in 4 cases (13%), and with RSV in 3 cases (10%; see Table 3 ). Sputum culture was positive in 54 cases (53% of available samples); however, only 101 participants (33%) could provide an adequate sample. In comparison, blood culture yielded relevant results in 16 (7%) of 231 cases. Polymerase chain reaction analysis of 201 available samples resulted in the identification of 43 (21%) atyp.....
    Document: Diarrhea 49 (16) 12 (20) 9 (25) 3 (14) 1 (13) with rhinovirus in 4 cases (13%), and with RSV in 3 cases (10%; see Table 3 ). Sputum culture was positive in 54 cases (53% of available samples); however, only 101 participants (33%) could provide an adequate sample. In comparison, blood culture yielded relevant results in 16 (7%) of 231 cases. Polymerase chain reaction analysis of 201 available samples resulted in the identification of 43 (21%) atypical bacterial pathogens and 47 (23%) viruses. In addition, 31 (25%) possible cases of S pneumoniae and 12 (12%) possible cases of H influenzae were detected using PCR. For S pneumoniae, 19 cases were not confirmed by other methods, whereas 4 cases of H influenzae were not confirmed by other methods. S pneumoniae and H influenzae were identified in 22 and 30 additional cases, respectively, using multiplex PCR, but these were above the cycle threshold and thus excluded from further analysis.

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