Author: Jo, Seri; Kim, Suwon; Shin, Dong Hae; Kim, Mi-Sun
Title: Inhibition of SARS-CoV 3CL protease by flavonoids Document date: 2019_11_14
ID: vynk8q8c_12
Snippet: To confirm the feasibility of the assay method independently, the fluorescence spectra from the tryptophan of the SARS-CoV 3CLpro with candidate inhibitors were investigated 20 . The fluorescence measurements were recorded with a SpectraMax i3x Multi-mode microplate reader (Molecular Devices) at excitation and emission wavelengths of 290 nm and 300-500 nm, respectively. The optimal excitation and emission wavelengths were determined by SoftMax Pr.....
Document: To confirm the feasibility of the assay method independently, the fluorescence spectra from the tryptophan of the SARS-CoV 3CLpro with candidate inhibitors were investigated 20 . The fluorescence measurements were recorded with a SpectraMax i3x Multi-mode microplate reader (Molecular Devices) at excitation and emission wavelengths of 290 nm and 300-500 nm, respectively. The optimal excitation and emission wavelengths were determined by SoftMax Pro. One tryptophan of the SARS-CoV 3CLpro showed a fluorescence emission with a peak at 350 nm after the excitation at the wavelength of 290 nm. In contrast, the flavonoids were almost non-fluorescent under the same experiment condition. Each 40 lM chemical was incubated with 1 lM the SARS-CoV 3CLpro for 1 h and the fluorescence intensity of the mixture was measured.
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