Author: Rathore, Shailendra S.; Liu, Yinghui; Yu, Haijia; Wan, Chun; Lee, MyeongSeon; Yin, Qian; Stowell, Michael H.B.; Shen, Jingshi
Title: Intracellular Vesicle Fusion Requires a Membrane-Destabilizing Peptide Located at the Juxtamembrane Region of the v-SNARE Document date: 2019_12_24
ID: pudp1eoo_33
Snippet: Microbial Strains-All the recombinant proteins in this study were expressed in E. Coli BL21 [B F − ompT hsdS(r B Vc peptide (residues 56-84 of VAMP2) were expressed and purified in E. coli using the pET28a vector Yu et al.,2018) . SNARE mutants were prepared similarly as the corresponding WT proteins. Full-length SNAREs were stored in a buffer containing 25 mM HEPES (pH 7.4), 400 mM KCl, 1% n-octyl-β-D-glucoside (OG), 10% glycerol, and 0.5 mM .....
Document: Microbial Strains-All the recombinant proteins in this study were expressed in E. Coli BL21 [B F − ompT hsdS(r B Vc peptide (residues 56-84 of VAMP2) were expressed and purified in E. coli using the pET28a vector Yu et al.,2018) . SNARE mutants were prepared similarly as the corresponding WT proteins. Full-length SNAREs were stored in a buffer containing 25 mM HEPES (pH 7.4), 400 mM KCl, 1% n-octyl-β-D-glucoside (OG), 10% glycerol, and 0.5 mM Tris(2-carboxyethyl)phosphine (TCEP). Soluble proteins were stored in a protein binding buffer (25 mM HEPES [pH 7.4], 150 mM KCl, 10% glycerol, and 0.5 mM TCEP).
Search related documents:
Co phrase search for related documents- corresponding wt protein and wt protein: 1, 2
- length snare and mm 10 glycerol: 1
- length snare and mm hepes: 1
- length snare and SNARE mutant: 1
- mm 10 glycerol and mm hepes: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14
- mm 10 glycerol and recombinant protein: 1
- mm 10 glycerol and soluble protein: 1
- mm 10 glycerol and wt protein: 1, 2
- mm hepes and recombinant protein: 1, 2, 3
- mm hepes and wt protein: 1
- recombinant protein and soluble protein: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- recombinant protein and study recombinant protein: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- recombinant protein and wt protein: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11
- soluble protein and study recombinant protein: 1
Co phrase search for related documents, hyperlinks ordered by date