Author: LI, Xunliang; LI, Pengchong; CAO, Liyan; BAI, Yunyun; CHEN, Huijie; LIU, He; REN, Xiaofeng; LI, Guangxing
Title: Porcine IL-12 plasmid as an adjuvant improves the cellular and humoral immune responses of DNA vaccine targeting transmissible gastroenteritis virus spike gene in a mouse model Document date: 2019_9_2
ID: rme6b022_26
Snippet: The sequences of pVAX1-(TGEV-S1) and pVAX1-(pIL-12) were analyzed from GenBank, and the results of nucleotide sequence comparison revealed 100 and 99.8% similarity with the strain accession numbers M94101 (PUR46-MAD) (S1) and NM_214013.1 (pIL-12), respectively. The pVAX1-(TGEV-S1) and pVAX1-(pIL-12) plasmids were successfully transfected and doi: 10.1292/jvms.18-0682 expressed in BHK-21 cells, as indicated from the results of the immunofluorescen.....
Document: The sequences of pVAX1-(TGEV-S1) and pVAX1-(pIL-12) were analyzed from GenBank, and the results of nucleotide sequence comparison revealed 100 and 99.8% similarity with the strain accession numbers M94101 (PUR46-MAD) (S1) and NM_214013.1 (pIL-12), respectively. The pVAX1-(TGEV-S1) and pVAX1-(pIL-12) plasmids were successfully transfected and doi: 10.1292/jvms.18-0682 expressed in BHK-21 cells, as indicated from the results of the immunofluorescence assay (Fig. 1) .
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