Author: SUZUKI, Kazuya; OGUMA, Keisuke; SENTSUI, Hiroshi
Title: Preparation of a cell line persistently infected with maedi/visna virus and production of viral antigens Document date: 2016_10_30
ID: tg4qupbm_4
Snippet: The cell suspension was seeded in a 6-well plastic plate and cultivated at 37°C for 1 day. After removal of the culture fluid, the cells were inoculated with 0.2 ml of cell-free infectious material (the culture fluid of FGL infected with MVV), incubated for 1 hr, and cultivated at 37°C in a maintenance medium, which contained a reduced FBS concentration (2%). These cells were cultivated for 7 days at the maximum, and the passages were repeated .....
Document: The cell suspension was seeded in a 6-well plastic plate and cultivated at 37°C for 1 day. After removal of the culture fluid, the cells were inoculated with 0.2 ml of cell-free infectious material (the culture fluid of FGL infected with MVV), incubated for 1 hr, and cultivated at 37°C in a maintenance medium, which contained a reduced FBS concentration (2%). These cells were cultivated for 7 days at the maximum, and the passages were repeated in T-25 tissue culture flasks. Approximately 10 6 cells and 100 μl of culture media were collected from each passage and subjected to PCR testing for proviral MVV.
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