Author: Pérez-Ruiz, Mercedes; Pedrosa-Corral, Irene; Sanbonmatsu-Gámez, Sara; Navarro-Marí, José-María
Title: Laboratory Detection of Respiratory Viruses by Automated Techniques Document date: 2012_11_30
ID: ted64zo4_13
Snippet: The availability of monoclonal antibodies has allowed the development of different technologies for detecting viral antigens from clinical samples. Fluorescent and chromatographic immunoassays are rapid, easy to perform and have proved their utility, mainly for RSV and Flu. The minimum (hands-on and hands-off) turnaround time that these techniques require for obtaining results have converted them as referral point-of-care diagnostic assays. Howev.....
Document: The availability of monoclonal antibodies has allowed the development of different technologies for detecting viral antigens from clinical samples. Fluorescent and chromatographic immunoassays are rapid, easy to perform and have proved their utility, mainly for RSV and Flu. The minimum (hands-on and hands-off) turnaround time that these techniques require for obtaining results have converted them as referral point-of-care diagnostic assays. However, sensitivity widely depends on the commercial method and on the type of patient and sample, ranging from 10% to 70% in the case of pandemic Flu [23] . Higher viral loads and thus, higher sensitivity of antigen detection methods for RSV and Flu have been observed in the paediatric population versus adults and in nasopharyngeal aspirates versus nasopharyngeal exudates [24, 25] .
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