Author: Dejnirattisai, Wanwisa; Webb, Andrew I.; Chan, Vera; Jumnainsong, Amonrat; Davidson, Andrew; Mongkolsapaya, Juthathip; Screaton, Gavin
Title: Lectin Switching During Dengue Virus Infection Document date: 2011_6_15
ID: qos9vu3r_6
Snippet: The dengue-2 strain, 16681, was grown in C6/36 cells, Vero cells, and monocyte-derived dendritic cells (DCs). Cell-free supernatants were used either neat or after concentration by ultracentrifugation at 45,000 rpm for 4 h at 4°C, and the virus pellet was resuspended in 1.5% fetal bovine serum (FBS)/ Leibovitz L-15. To concentrate large volumes of low-titer DENV supernatant, DENV were precipitated with 10% Polyethylene glycol 8000 (Sigma) before.....
Document: The dengue-2 strain, 16681, was grown in C6/36 cells, Vero cells, and monocyte-derived dendritic cells (DCs). Cell-free supernatants were used either neat or after concentration by ultracentrifugation at 45,000 rpm for 4 h at 4°C, and the virus pellet was resuspended in 1.5% fetal bovine serum (FBS)/ Leibovitz L-15. To concentrate large volumes of low-titer DENV supernatant, DENV were precipitated with 10% Polyethylene glycol 8000 (Sigma) before ultracentrifugation. U937 were maintained in 10% FBS/Roswell Park Memorial Institute medium (RPMI). NIH/3T3, and DC-SIGN and L-SIGN NIH/3T3 cell lines were obtained through the AIDS Research and Reference Reagent Program (Division of AIDS, National Institute of Allergy and Infectious Diseases, from Drs Thomas D. Martin and Vineet N. Kewal Ramani) and maintained in 10% FBS/ Dulbecco's Modified Eagle's medium. All media were supplemented with 2 mmol/L L-glutamine, 100 U/mL penicillin, and 100 U/mL streptomycin.
Search related documents:
Co phrase search for related documents- bovine serum and Dulbecco Modified Eagle medium: 1, 2, 3, 4, 5
- bovine serum and FBS Roswell Park Memorial Institute medium: 1, 2
- bovine serum and fetal bovine serum: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84
- bovine serum and large volume: 1
- bovine serum and Leibovitz fetal bovine serum: 1
- bovine serum and low titer: 1
- bovine serum and Polyethylene glycol: 1, 2, 3, 4, 5, 6
- bovine serum and Vero cell: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13
- bovine serum and virus pellet: 1
- cell line and dendritic cell: 1, 2, 3, 4, 5
- cell line and DENV supernatant: 1
- cell line and Dulbecco Modified Eagle medium: 1
- cell line and fetal bovine serum: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48
- cell line and Leibovitz fetal bovine serum: 1
- cell line and low titer: 1
- cell line and Polyethylene glycol: 1, 2, 3
- cell line and SIGN cell line: 1, 2, 3
- cell line and Vero cell: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75
- dendritic cell and large volume: 1
Co phrase search for related documents, hyperlinks ordered by date