Selected article for: "Golgi localization and protein retention"
Title: Localization and targeting of the Saccharomyces cerevisiae Kre2p/Mnt1p alpha 1,2-mannosyltransferase to a medial-Golgi compartment
  • Document date: 1995_11_2
    • ID: q1jx0n0l_43
    Snippet: Lastly, to assess if the Kre2p TMD is required for proper Golgi retention in the context of an heterologous luminal domain, we constructed fusion protein KPKP in which the Kre2p TMD of KKKP was removed and substituted with that of PhoSp (see Fig. 6 ). Golgi retention of this fusion protein would indicate that the Kre2p NH2 terminus cytoplasmic and stem domains are sufficient to achieve correct Golgi intracellular localization. Immunofluorescence .....
    Document: Lastly, to assess if the Kre2p TMD is required for proper Golgi retention in the context of an heterologous luminal domain, we constructed fusion protein KPKP in which the Kre2p TMD of KKKP was removed and substituted with that of PhoSp (see Fig. 6 ). Golgi retention of this fusion protein would indicate that the Kre2p NH2 terminus cytoplasmic and stem domains are sufficient to achieve correct Golgi intracellular localization. Immunofluorescence studies of KPKP revealed that this chimeric protein was retained in the ER (data not shown), and thus uninformative.

    Search related documents:
    Co phrase search for related documents
    • chimeric protein ER retain and Golgi retention: 1
    • ER retain and fusion protein: 1
    • ER retain and Golgi retention: 1, 2
    • fusion protein and Golgi retention: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18
    • fusion protein and heterologous luminal domain: 1
    • fusion protein and immunofluorescence study: 1, 2
    • fusion protein and intracellular localization: 1, 2, 3, 4, 5, 6
    • fusion protein and luminal domain: 1, 2, 3, 4, 5, 6
    • fusion protein and stem cytoplasmic domain: 1
    • fusion protein Golgi retention and Golgi retention: 1, 2
    • Golgi intracellular localization and intracellular localization: 1, 2, 3, 4
    • Golgi retention and heterologous luminal domain: 1
    • Golgi retention and intracellular localization: 1, 2, 3, 4, 5, 6, 7, 8, 9
    • Golgi retention and luminal domain: 1, 2, 3, 4, 5, 6, 7, 8
    • Golgi retention and stem cytoplasmic domain: 1, 2
    • heterologous luminal domain and intracellular localization: 1
    • heterologous luminal domain and luminal domain: 1, 2
    • intracellular localization and luminal domain: 1, 2
    • luminal domain and stem cytoplasmic domain: 1, 2