Selected article for: "chimeric protein and fusion protein"
Title: Localization and targeting of the Saccharomyces cerevisiae Kre2p/Mnt1p alpha 1,2-mannosyltransferase to a medial-Golgi compartment
  • Document date: 1995_11_2
    • ID: q1jx0n0l_43
    Snippet: Lastly, to assess if the Kre2p TMD is required for proper Golgi retention in the context of an heterologous luminal domain, we constructed fusion protein KPKP in which the Kre2p TMD of KKKP was removed and substituted with that of PhoSp (see Fig. 6 ). Golgi retention of this fusion protein would indicate that the Kre2p NH2 terminus cytoplasmic and stem domains are sufficient to achieve correct Golgi intracellular localization. Immunofluorescence .....
    Document: Lastly, to assess if the Kre2p TMD is required for proper Golgi retention in the context of an heterologous luminal domain, we constructed fusion protein KPKP in which the Kre2p TMD of KKKP was removed and substituted with that of PhoSp (see Fig. 6 ). Golgi retention of this fusion protein would indicate that the Kre2p NH2 terminus cytoplasmic and stem domains are sufficient to achieve correct Golgi intracellular localization. Immunofluorescence studies of KPKP revealed that this chimeric protein was retained in the ER (data not shown), and thus uninformative.

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