Author: Imai-Matsushima, Aki; Martin-Sancho, Laura; Karlas, Alexander; Imai, Seiichiro; Zoranovic, Tamara; Hocke, Andreas C.; Mollenkopf, Hans-Joachim; Berger, Hilmar; Meyer, Thomas F.
Title: Long-Term Culture of Distal Airway Epithelial Cells Allows Differentiation Towards Alveolar Epithelial Cells Suited for Influenza Virus Studies Document date: 2018_6_22
ID: vopbw1tl_6
Snippet: The result is a novel method that allows expansion of human DAECs using feeder cells. Feeder removal induced a strong inflammatory response and differentiation into an airway club cell phenotype. Addition of small molecules and growth factors at the end of the expansion phase induced differentiation into AEII cells, followed by trans-differentiation into type I cells. We successfully adapted this method to chicken DAECs and compared the growth ki.....
Document: The result is a novel method that allows expansion of human DAECs using feeder cells. Feeder removal induced a strong inflammatory response and differentiation into an airway club cell phenotype. Addition of small molecules and growth factors at the end of the expansion phase induced differentiation into AEII cells, followed by trans-differentiation into type I cells. We successfully adapted this method to chicken DAECs and compared the growth kinetics of different IAV strains between the two species. Additionally, our model supports siRNA transfection, enabling the application of advanced molecular techniques on primary DAECs to allow physiologically relevant research on various human and zoonotic lung diseases.
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