Author: Ma, J; Wan, J; Meng, J; Banerjee, S; Ramakrishnan, S; Roy, S
Title: Methamphetamine induces autophagy as a pro-survival response against apoptotic endothelial cell death through the Kappa opioid receptor Document date: 2014_3_6
ID: wu2mogfa_26
Snippet: Transfection with siRNA. ON-TARGET plus SMART Pool Human Beclin1 siRNA (L-010552-00) and human KOR siRNA (L-005684-00) were used as per the manufacturer's instructions. Transfections were carried out as previously described. 41 Scrambled siRNA was used as a negative control. The reduction in Beclin1 and KOR mRNA expression were estimated by real-time PCR analysis. Caspase-3/7 activity was analyzed using Caspase-Glo luminescent-based assays accord.....
Document: Transfection with siRNA. ON-TARGET plus SMART Pool Human Beclin1 siRNA (L-010552-00) and human KOR siRNA (L-005684-00) were used as per the manufacturer's instructions. Transfections were carried out as previously described. 41 Scrambled siRNA was used as a negative control. The reduction in Beclin1 and KOR mRNA expression were estimated by real-time PCR analysis. Caspase-3/7 activity was analyzed using Caspase-Glo luminescent-based assays according to the manufacturer's instructions (Promega Corporation).
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