Author: Gunn, Michael D.; Kyuwa, Shigeru; Tam, Carmen; Kakiuchi, Terutaka; Matsuzawa, Akio; Williams, Lewis T.; Nakano, Hideki
Title: Mice Lacking Expression of Secondary Lymphoid Organ Chemokine Have Defects in Lymphocyte Homing and Dendritic Cell Localization Document date: 1999_2_1
ID: sz28ar3t_15
Snippet: Lack of SLC Expression in plt Mice. The expression pattern of SLC mRNA and protein in normal mice has been determined (22, 23) . To examine the expression of SLC in plt mice, in situ hybridization on tissue sections was performed using 35 S-labeled SLC antisense riboprobe. No SLC mRNA was detected in any tissue of plt mice, includ- ing LN, spleen, PP, thymus, and lymphatic endothelium, whereas the location and abundance of SLC mRNA was normal in .....
Document: Lack of SLC Expression in plt Mice. The expression pattern of SLC mRNA and protein in normal mice has been determined (22, 23) . To examine the expression of SLC in plt mice, in situ hybridization on tissue sections was performed using 35 S-labeled SLC antisense riboprobe. No SLC mRNA was detected in any tissue of plt mice, includ- ing LN, spleen, PP, thymus, and lymphatic endothelium, whereas the location and abundance of SLC mRNA was normal in the same tissues of Ï©/Ï© mice (Fig. 1 , A-F, and data not shown). Northern blot analysis of total RNA from peripheral LNs and spleen confirmed the complete absence of SLC transcripts in plt mice (Fig. 1 G) .
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