Author: Chow, Ken Yan Ching; Hon, Chung Chau; Hui, Raymond Kin Hi; Wong, Raymond Tsz Yeung; Yip, Chi Wai; Zeng, Fanya; Leung, Frederick Chi Ching
Title: Molecular Advances in Severe Acute Respiratory Syndrome-associated Coronavirus (SARS-CoV) Document date: 2016_11_28
ID: xuj4yymz_48
Snippet: 2 ) and vero cells (3 ) were found to be susceptible to SARS-CoV infection. After the isolation procedure, the pathogen was identified as the SARS-CoV by further tests, such as electron microscopy, RT-PCR, or immunofluorescent viral antigen detection. Virus isolation is the only means to detect the existence of live virus from the tissue. The methodology is generally employed only for a preliminary identification of an unknown pathogen, as the pr.....
Document: 2 ) and vero cells (3 ) were found to be susceptible to SARS-CoV infection. After the isolation procedure, the pathogen was identified as the SARS-CoV by further tests, such as electron microscopy, RT-PCR, or immunofluorescent viral antigen detection. Virus isolation is the only means to detect the existence of live virus from the tissue. The methodology is generally employed only for a preliminary identification of an unknown pathogen, as the procedure requires skillful technicians and is time consuming. The requirement of infectious viruses and that the duration of live virus existence varies add on further problems for conducting such assays, but they are nevertheless of very high specificity.
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