Title: A Golgi retention signal in a membrane-spanning domain of coronavirus E1 protein Document date: 1991_10_1
ID: s4a8zs5a_15
Snippet: Radiolabeling and Immunoprecipitation COS-7 cells expressing El and mutant El proteins, or G and mutant G proteins were labeled -44 h posttransfection . El proteins were labeled for 1 or 2 h in 0.5 ml cysteine-free DME with 100 ACi [35 8 ]cysteine (1,300 Ci/ mmol ; Amersham Corp ., Arlington Heights, IL) . Cells were harvested immediately, or after a 3 h chase in regular growth medium containing a threefold excess of unlabeled cysteine. Cells wer.....
Document: Radiolabeling and Immunoprecipitation COS-7 cells expressing El and mutant El proteins, or G and mutant G proteins were labeled -44 h posttransfection . El proteins were labeled for 1 or 2 h in 0.5 ml cysteine-free DME with 100 ACi [35 8 ]cysteine (1,300 Ci/ mmol ; Amersham Corp ., Arlington Heights, IL) . Cells were harvested immediately, or after a 3 h chase in regular growth medium containing a threefold excess of unlabeled cysteine. Cells were lysed in detergent solution (50 mM Tris, pH 8.0, 1% NP-40,0.4% deoxycholate, 62 .5 mM EDTA, and 0.13 TIU/ml aprotinin) and El proteins immunoprecipitated using the anti-peptide serum and fixed Staphylococcus aureus (Calbiochem-Behring Corp., San Diego, CA) as described previously (26) .
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