Author: Zhou, Yan; Zheng, HaiHong; Gao, Fei; Tian, DeBin; Yuan, ShiShan
Title: Mutational analysis of the SDD sequence motif of a PRRSV RNA-dependent RNA polymerase Document date: 2011_9_16
ID: yo9libo0_35
Snippet: To analyze the viral RNA profiles, total RNAs were isolated at the 24 h postinfection from MARC-145 cells infected by the P5 mutant viruses (vGDD) and P5 vAPPRS at an MOI of 1. The RNAs were separated on formaldehyde-denatured agarose gel and subjected to Northern blotting with a DIG-labeled probe complementary to the N protein sequence of type II PRRSV. The Northern blot results revealed that the sg mRNAs of mutants could be recognized by the sp.....
Document: To analyze the viral RNA profiles, total RNAs were isolated at the 24 h postinfection from MARC-145 cells infected by the P5 mutant viruses (vGDD) and P5 vAPPRS at an MOI of 1. The RNAs were separated on formaldehyde-denatured agarose gel and subjected to Northern blotting with a DIG-labeled probe complementary to the N protein sequence of type II PRRSV. The Northern blot results revealed that the sg mRNAs of mutants could be recognized by the specific probe and that the sg mRNA5 was the most abundant RNA in the virus transcription process (Figure 4) . Compared with vAPRRS, the transcription pattern in the mutant virus vGDD was altered as a result of the change to the motif (Figure 4 ).
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