Next-Generation Sequencing Reveals HIV-1-Mediated Suppression of T Cell Activation and RNA Processing and Regulation of Noncoding RNA Expression in a CD4(+) T Cell Line

Chang, Stewart T.; Sova, Pavel; Peng, Xinxia; Weiss, Jeffrey; Law, G. Lynn; Palermo, Robert E.; Katze, Michael G.

Many of these cellular responses are also reflected at the level of the host organism. For example, gene expression in the lymph nodes of simian immunodeficiency virus (SIV)-infected Asian pig-tailed macaques (a model of pathogenic HIV infection) reflects strong and sustained type I interferon responses (6) . A sim-ilar initial interferon response is seen in the natural host, African green monkeys, but it eventually subsides and the infection resolves (6) . Despite intense investigation into the molecular events following infection in these and other studies, fundamental gaps in knowledge still remain. For instance, the extent to which host and viral gene expression respond to each other is still poorly understood. New technologies enabling the measurement of both host and viral RNA may help to address such shortcomings.