Selected article for: "complete genome and genome sequencing"
Author: Wang, Yanqun; Liu, Di; Shi, Weifeng; Lu, Roujian; Wang, Wenling; Zhao, Yanjie; Deng, Yao; Zhou, Weimin; Ren, Hongguang; Wu, Jun; Wang, Yu; Wu, Guizhen; Gao, George F.; Tan, Wenjie
Title: Origin and Possible Genetic Recombination of the Middle East Respiratory Syndrome Coronavirus from the First Imported Case in China: Phylogenetics and Coalescence Analysis
  • Document date: 2015_9_8
    • ID: x6sjdglm_11
    Snippet: Full-length genomic sequencing. Nasopharyngeal swabs from the South Korean patient diagnosed with MERS-CoV infection were collected and used for viral RNA extraction with the QIAamp viral RNA minikit. Fortyfour sets of specific primer pairs were designed and used to amplify the complete genome, followed by Sanger sequencing; meanwhile, the extracted viral RNA was also used for next-generation sequencing with the Ion Torrent PGM after random ampli.....
    Document: Full-length genomic sequencing. Nasopharyngeal swabs from the South Korean patient diagnosed with MERS-CoV infection were collected and used for viral RNA extraction with the QIAamp viral RNA minikit. Fortyfour sets of specific primer pairs were designed and used to amplify the complete genome, followed by Sanger sequencing; meanwhile, the extracted viral RNA was also used for next-generation sequencing with the Ion Torrent PGM after random amplification.

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