Selected article for: "agarose gel and forward primer"
Author: Mohamed, Fakry F.; Ktob, Gamelat K.F.; Ismaeil, Mohamed E.A.; Ali, Ahmed A.H.; Goyal, Sagar M.
Title: Phylogeny of bovine norovirus in Egypt based on VP2 gene
  • Document date: 2018_4_13
    • ID: rah5p1y4_17
    Snippet: Using the RT-PCR with BNoV-RdRp specific primers, 8 of 29 fecal samples (27.6%) were confirmed positive for BNoV-RNA. Positive samples showed specific bands of 532 bp on 1.5% agarose gel under UV rays (Fig. 1) . The previous step aimed to rule out the negative samples for BNoV as the RdRp is highly conserved and commonly used for BNoV detection. Furthermore, positive RNA samples were retested by RT-PCR but with BNoV-VP2 targeting primers in order.....
    Document: Using the RT-PCR with BNoV-RdRp specific primers, 8 of 29 fecal samples (27.6%) were confirmed positive for BNoV-RNA. Positive samples showed specific bands of 532 bp on 1.5% agarose gel under UV rays (Fig. 1) . The previous step aimed to rule out the negative samples for BNoV as the RdRp is highly conserved and commonly used for BNoV detection. Furthermore, positive RNA samples were retested by RT-PCR but with BNoV-VP2 targeting primers in order to characterize the VP2 gene molecularly. The designed primers annealed successively to their sites; nt 6432-6449 on the plus strand for the forward primer and nt 7257-7237 on the minus strand for the reverse primer and the expected bands (826 bp) were demonstrated on the gel. Samples tested negative for BRV, BVDV, and bovine coronavirus (data not shown).

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