Author: LI, Xunliang; LI, Pengchong; CAO, Liyan; BAI, Yunyun; CHEN, Huijie; LIU, He; REN, Xiaofeng; LI, Guangxing
Title: Porcine IL-12 plasmid as an adjuvant improves the cellular and humoral immune responses of DNA vaccine targeting transmissible gastroenteritis virus spike gene in a mouse model Document date: 2019_9_2
ID: rme6b022_15
Snippet: Peripheral blood MCs (PBMCs) and splenic MCs (SMCs) were resuspended at 1 × 10 7 cells/ml and incubated with either phycoerythrin (PE)-labeled anti-porcine CD8 + or fluorescein isothiocyanate (FITC)-labeled anti-porcine CD4 + antibodies (1:1,000 diluted in PBS; Zhongshan, Guangdong, China). The frequencies of CD4 + and CD8 + cells were quantified with flow cytometric analysis (BD, Franklin Lakes, NJ, U.S.A.)......
Document: Peripheral blood MCs (PBMCs) and splenic MCs (SMCs) were resuspended at 1 × 10 7 cells/ml and incubated with either phycoerythrin (PE)-labeled anti-porcine CD8 + or fluorescein isothiocyanate (FITC)-labeled anti-porcine CD4 + antibodies (1:1,000 diluted in PBS; Zhongshan, Guangdong, China). The frequencies of CD4 + and CD8 + cells were quantified with flow cytometric analysis (BD, Franklin Lakes, NJ, U.S.A.).
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