Author: SUZUKI, Kazuya; OGUMA, Keisuke; SENTSUI, Hiroshi
Title: Preparation of a cell line persistently infected with maedi/visna virus and production of viral antigens Document date: 2016_10_30
ID: tg4qupbm_6
Snippet: According to the information (cell line details) about ZZ-R documented by Friedrich-Loeffler-Institut, which provided ZZ-R cells, the cells are free of contamination by bacteria, Mycoplasma, fungi and viruses, such as bovine herpesvirus 1 and 4, bovine and porcine rotavirus type A, bovine coronavirus, bovine respiratory syncytial virus, bovine parainfluenza virus 3, bovine leukemia virus, bovine rotavirus type 1, bovine and porcine adenoviruses, .....
Document: According to the information (cell line details) about ZZ-R documented by Friedrich-Loeffler-Institut, which provided ZZ-R cells, the cells are free of contamination by bacteria, Mycoplasma, fungi and viruses, such as bovine herpesvirus 1 and 4, bovine and porcine rotavirus type A, bovine coronavirus, bovine respiratory syncytial virus, bovine parainfluenza virus 3, bovine leukemia virus, bovine rotavirus type 1, bovine and porcine adenoviruses, BVDV, classical fever virus, transmissible gastroenteritis virus, porcine parvovirus and porcine enterovirus. No viral particles were detected in the cells by repeated electron microscopy examinations. We also tried to rule out the contamination with mycoplasma and BVDV, which are often detected in cultivated cells. The cell pellets were subjected to PCR testing for both agents [7, 17] and found to be free of them. Therefore, we believe that a cell line persistently producing MVV and at least free of the contamination with mycoplasma and BVDV has been established.
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