Selected article for: "cell culture and real time"

Author: Huang, G Khai Lin; Tio, Shio Yen; Caly, Leon; Nicholson, Suellen; Thevarajan, Irani; Papadakis, Georgina; Catton, Mike; Tong, Steven Y C; Druce, Julian
Title: Prolonged Detection of Japanese Encephalitis Virus in Urine and Whole Blood in a Returned Short-term Traveler
  • Document date: 2017_9_27
  • ID: ymcgs03n_6
    Snippet: Aliquots of urine and whole blood were inoculated onto cultured cells to assess viral infectivity (see the Supplementary Data for detailed methods). Cytopathic effects were observed 7 days postinoculation from the day 14 urine specimen. The cell culture supernatant was tested by JEV real-time PCR and was found to be positive with a high level of detection, suggesting efficient viral replication......
    Document: Aliquots of urine and whole blood were inoculated onto cultured cells to assess viral infectivity (see the Supplementary Data for detailed methods). Cytopathic effects were observed 7 days postinoculation from the day 14 urine specimen. The cell culture supernatant was tested by JEV real-time PCR and was found to be positive with a high level of detection, suggesting efficient viral replication.

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