Author: Tan, Zhaoli; Gao, Lihua; Wang, Yan; Yin, Huihui; Xi, Yongyi; Wu, Xiaojie; Shao, Yong; Qiu, Weiyi; Du, Peng; Shen, Wenlong; Fu, Ling; Jia, Ru; Zhao, Chuanhua; Zhang, Yun; Zhao, Zhihu; Sun, Zhiwei; Chen, Hongxing; Hu, Xianwen; Xu, Jianming; Wang, Youliang
Title: PRSS contributes to cetuximab resistance in colorectal cancer Document date: 2020_1_1
ID: tymoeyoo_64
Snippet: Serum or cell culture supernatant cetuximab concentrations were detected using a validated sandwich ELISA. Briefly, EGFR-coated polystyrene microtiter plates were loaded with serum samples, washed, and incubated with an HRP-conjugated goat anti-human IgG (Sigma-Aldrich) or an HRP-conjugated goat anti-mouse IgG (Pierce) . After elution of the unbound conjugate, the chromogenic substrate was added, and the colored reaction product was measured usin.....
Document: Serum or cell culture supernatant cetuximab concentrations were detected using a validated sandwich ELISA. Briefly, EGFR-coated polystyrene microtiter plates were loaded with serum samples, washed, and incubated with an HRP-conjugated goat anti-human IgG (Sigma-Aldrich) or an HRP-conjugated goat anti-mouse IgG (Pierce) . After elution of the unbound conjugate, the chromogenic substrate was added, and the colored reaction product was measured using a microtiter plate reader at 450 nm/540 nm. Calibration curves were linear in the concentration range of 0.475 to 14.25 ïg/ml. Pharmacokinetic variables were calculated, and data on assay specificity, linearity, precision, and accuracy and the limit of quantification were obtained.
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