Author: Nordén, Rickard; Magnusson, Jesper; Lundin, Anna; Tang, Ka-Wei; Nilsson, Staffan; Lindh, Magnus; Andersson, Lars-Magnus; Riise, Gerdt C; Westin, Johan
Title: Quantification of Torque Teno Virus and Epstein-Barr Virus Is of Limited Value for Predicting the Net State of Immunosuppression After Lung Transplantation Document date: 2018_3_6
ID: zhlvvuj4_16
Snippet: TTV load was quantified on the QX200 Droplet Digital PCR system using the ddPCR supermix for probes (No dUTP; Bio-Rad, Hercules, CA). Primer and probe concentrations in the 20-µL final reaction were 300 and 200 nM, respectively. The PCR conditions were 50°C for 2 minutes followed by 95°C for 10 minutes, then 30 seconds at 95°C and 1 minute at 58°C for a total of 40 cycles, and finally a step at 98°C for 10 minutes. After overnight incubatio.....
Document: TTV load was quantified on the QX200 Droplet Digital PCR system using the ddPCR supermix for probes (No dUTP; Bio-Rad, Hercules, CA). Primer and probe concentrations in the 20-µL final reaction were 300 and 200 nM, respectively. The PCR conditions were 50°C for 2 minutes followed by 95°C for 10 minutes, then 30 seconds at 95°C and 1 minute at 58°C for a total of 40 cycles, and finally a step at 98°C for 10 minutes. After overnight incubation at 4°C, the droplet fluorescence signal was determined. Data analysis was done using Bio-Rad Quanta Soft analysis software.
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