Author: Rogers, J.; Schoepp, R.J.; Schröder, O.; Clements, T.L.; Holland, T.F.; Li, J.Q.; Li, J.; Lewis, L.M.; Dirmeier, R.P.; Frey, G.J.; Tan, X.; Wong, K.; Woodnutt, G.; Keller, M.; Reed, D.S.; Kimmel, B.E.; Tozer, E.C.
Title: Rapid discovery and optimization of therapeutic antibodies against emerging infectious diseases Document date: 2008_5_13
ID: xkx56h0o_5
Snippet: The library plasmid pBAD_ZF was constructed in several steps using pBAD/gIII (Invitrogen, Carlsbad, CA, USA) as the starting plasmid. A cassette containing a polynucleotide encoding a zinc finger protein, a Flag tag, and a His tag was inserted into the NdeI/PmeI site. Next, the zinc finger binding domain sequence was inserted into the plasmid after the terminator by QuikChange TM site-directed mutagenesis (Stratagene, La Jolla, CA, USA). The fina.....
Document: The library plasmid pBAD_ZF was constructed in several steps using pBAD/gIII (Invitrogen, Carlsbad, CA, USA) as the starting plasmid. A cassette containing a polynucleotide encoding a zinc finger protein, a Flag tag, and a His tag was inserted into the NdeI/PmeI site. Next, the zinc finger binding domain sequence was inserted into the plasmid after the terminator by QuikChange TM site-directed mutagenesis (Stratagene, La Jolla, CA, USA). The final product vector, pBAD_ZF, was used for cloning and expression of antibody light and heavy chains, as described below.
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