Selected article for: "assay sensitivity and detection limit"
Author: Neethirajan, Suresh; Ahmed, Syed Rahin; Chand, Rohit; Buozis, John; Nagy, Éva
Title: Recent Advances in Biosensor Development for Foodborne Virus Detection
  • Document date: 2017_7_5
    • ID: sj6zfybb_59
    Snippet: Traditional lateral-flow assay (LFA) has the drawback of lower sensitivity due to its colored particles, such as gold nanoparticles or blue latex. A novel LFA using phage nanoparticles as reporters is used to detect norovirus-like particles based on an optimized antibody sandwich pair identified from ELISA [56] . The use of the antibody pair is validated by both gold nanoparticle and bacteriophage and LFA. The phage nanoparticle LFA is rapid and .....
    Document: Traditional lateral-flow assay (LFA) has the drawback of lower sensitivity due to its colored particles, such as gold nanoparticles or blue latex. A novel LFA using phage nanoparticles as reporters is used to detect norovirus-like particles based on an optimized antibody sandwich pair identified from ELISA [56] . The use of the antibody pair is validated by both gold nanoparticle and bacteriophage and LFA. The phage nanoparticle LFA is rapid and highly sensitive with a detection limit of 107 virus copies/mL, which is 100-fold lower than the gold nanoparticle LFA alone with the same antibody pair.

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