Author: Tang, Hei-Man Vincent; Gao, Wei-Wei; Chan, Chi-Ping; Cheng, Yun; Chaudhary, Vidyanath; Deng, Jian-Jun; Yuen, Kit-San; Wong, Chun-Ming; Ng, Irene Oi-Lin; Kok, Kin-Hang; Zhou, Jie; Jin, Dong-Yan
Title: Requirement of CRTC1 coactivator for hepatitis B virus transcription Document date: 2014_11_10
ID: qtoygz6w_20
Snippet: Real-time reverse transcription-PCR (RT-PCR) was performed as described (27, 30) . Normalization was made to glycerialdehyde-3-phosphate dehydrogenase (GAPDH) mRNA. Quantitation of target mRNA was achieved with the comparative Ct method. Relative expression level of target mRNA was calculated from 2 − Ct . Primers were listed in Supplementary Table S1 ......
Document: Real-time reverse transcription-PCR (RT-PCR) was performed as described (27, 30) . Normalization was made to glycerialdehyde-3-phosphate dehydrogenase (GAPDH) mRNA. Quantitation of target mRNA was achieved with the comparative Ct method. Relative expression level of target mRNA was calculated from 2 − Ct . Primers were listed in Supplementary Table S1 .
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