Title: Research Communications of the 27(th) ECVIM-CA Congress: Intercontinental, Saint Julian's, Malta, 14th to 16th September 2017 Document date: 2017_11_7
ID: roslkxeq_162
Snippet: Canine primary adrenocortical cell cultures from adrenal glands of seven healthy dogs were incubated with various concentrations of AA. PDH was mimicked by co-incubation with synthetic ACTH (SynacthenÃ’). After 48 h of incubation, RNA was isolated to evaluate gene expression using real-time quantitative PCR (RT-qPCR). Culture medium was removed for measurement of cortisol after 72 h of incubation. In addition, cell viability was assessed using al.....
Document: Canine primary adrenocortical cell cultures from adrenal glands of seven healthy dogs were incubated with various concentrations of AA. PDH was mimicked by co-incubation with synthetic ACTH (SynacthenÃ’). After 48 h of incubation, RNA was isolated to evaluate gene expression using real-time quantitative PCR (RT-qPCR). Culture medium was removed for measurement of cortisol after 72 h of incubation. In addition, cell viability was assessed using alamarBlueÃ’ assay.
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