Selected article for: "negative control and primary antibody"

Title: Research Communications of the 27(th) ECVIM-CA Congress: Intercontinental, Saint Julian's, Malta, 14th to 16th September 2017
  • Document date: 2017_11_7
  • ID: roslkxeq_262
    Snippet: Western blot analysis showed the primary antibody bound a single antigen of 120-130 kDa in size, which is the appropriate molecular size for a-klotho. No positive staining was detected in the negative isotype control sections, confirming the specificity of the staining for a-klotho. Alpha-klotho protein expression was identified immunohistochemically both in the proximal and distal tubules of murine and feline kidney tissue, with more intense sta.....
    Document: Western blot analysis showed the primary antibody bound a single antigen of 120-130 kDa in size, which is the appropriate molecular size for a-klotho. No positive staining was detected in the negative isotype control sections, confirming the specificity of the staining for a-klotho. Alpha-klotho protein expression was identified immunohistochemically both in the proximal and distal tubules of murine and feline kidney tissue, with more intense staining of the distal tubules compared to the proximal tubules.

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