Retention of p63 in an ER-Golgi intermediate compartment depends on the presence of all three of its domains and on its ability to form oligomers

P63 Cytoplasmic Tail Mutants. The p63wt cDNA was as described previously (Schweizer et al., 1993b) and consisted of the 5' untranslated region, bpl-84, the 1803 nucleotide coding region and 1023 bp of the 3' noncoding sequence. The full-length cDNA was inserted into the EcoRI site in the polylinker of the Bluescript SK-or KS-vector (Stratagene, La Jolla, CA), respectively, with the initiator ATG facing the BamHI restriction site of the polylinker. The resulting constructs were designated pBSK-p63 or pBKS-p63, respectively. For transient expression in COS cells, the p63 insert was subcloned into the EcoRI site of the pECE vector (kindly provided by Dr. M. Spiess, Biozentrum, Basel, Switzerland) (Ellis ct al., 1986) to give plasmid pECE-p63.