Title: Retention of p63 in an ER-Golgi intermediate compartment depends on the presence of all three of its domains and on its ability to form oligomers Document date: 1994_7_1
ID: ra20actc_14
Snippet: To generate the A2-101 construct (p63 with deletion of amino acids 2-101), pBSK-p63 as template together with the 5' primer C-CC CGC CAT GCG CAG GCT CGG CAG GC~ G-CT CAA C, and a 3' primer corresponding to nucleotides 774-791 of the p63wt sequence were used in the PCR reaction. In a subsequent PCR reaction, the final A2-101 construct and the 5' primer AAG GAT CCG AAT TCG CCC GCC ATG GCT GCA CTC AGG GCG CTC AAC TIT were used to produce the A2-101 .....
Document: To generate the A2-101 construct (p63 with deletion of amino acids 2-101), pBSK-p63 as template together with the 5' primer C-CC CGC CAT GCG CAG GCT CGG CAG GC~ G-CT CAA C, and a 3' primer corresponding to nucleotides 774-791 of the p63wt sequence were used in the PCR reaction. In a subsequent PCR reaction, the final A2-101 construct and the 5' primer AAG GAT CCG AAT TCG CCC GCC ATG GCT GCA CTC AGG GCG CTC AAC TIT were used to produce the A2-101 AA mutant.
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