Author: Yu Jin Jung; Gun-Soo Park; Jun Hye Moon; Keunbon Ku; Seung-Hwa Beak; Seil Kim; Edmond Changkyun Park; Daeui Park; Jong-Hwan Lee; Cheol Woo Byeon; Joong Jin Lee; Jin-Soo Maeng; Seong Jun Kim; Seung Il Kim; Bum-Tae Kim; Min Jun Lee; Hong Gi Kim
Title: Comparative analysis of primer-probe sets for the laboratory confirmation of SARS-CoV-2 Document date: 2020_2_27
ID: 2hyiped2_8
Snippet: The infection experiments were performed in a biosafety level-3 (BSL-3) laboratory. African green monkey kidney Vero cells (ATCC CCL-81) were infected with a clinical isolate SARS-CoV-2 (BetaCoV/Korea/KCDC03/2020 provided from Korea CDC). After 72 h, the culture medium containing mature infectious virions (virus medium) was collected and viral RNA was isolated from the culture medium using the QIAamp viral RNA extraction Kit (Qiagen, Hilden, Germ.....
Document: The infection experiments were performed in a biosafety level-3 (BSL-3) laboratory. African green monkey kidney Vero cells (ATCC CCL-81) were infected with a clinical isolate SARS-CoV-2 (BetaCoV/Korea/KCDC03/2020 provided from Korea CDC). After 72 h, the culture medium containing mature infectious virions (virus medium) was collected and viral RNA was isolated from the culture medium using the QIAamp viral RNA extraction Kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions.
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