Author: Wang, Jiying; Wang, Yanping; Wang, Huaizhong; Hao, Xiaojing; Wu, Ying; Guo, Jianfeng
Title: Selection of Reference Genes for Gene Expression Studies in Porcine Whole Blood and Peripheral Blood Mononuclear Cells under Polyinosinic:Polycytidylic Acid Stimulation Document date: 2014_4_23
ID: xf4yy6i1_20
Snippet: Using the mix pool RNA of the PBMC and whole blood samples, we generated amplification efficiency and melting curve analyses for the primers of the six candidate reference genes, and their results were shown in the last column of Figure S1 (See e-version for supplement.), respectively. The amplification efficiencies for the six candidate reference genes ranged between 95.3% and 109.30%. The melting curves of the six pairs of primers were single, .....
Document: Using the mix pool RNA of the PBMC and whole blood samples, we generated amplification efficiency and melting curve analyses for the primers of the six candidate reference genes, and their results were shown in the last column of Figure S1 (See e-version for supplement.), respectively. The amplification efficiencies for the six candidate reference genes ranged between 95.3% and 109.30%. The melting curves of the six pairs of primers were single, smooth and sharply defined with a narrow peak, suggesting all the six pairs of primers produce pure and homogeneous products. Thus, all the primers for the six candidate reference gene were specific and suitable for further qRT-PCR assays.
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