Author: Wang, Jiying; Wang, Yanping; Wang, Huaizhong; Hao, Xiaojing; Wu, Ying; Guo, Jianfeng
Title: Selection of Reference Genes for Gene Expression Studies in Porcine Whole Blood and Peripheral Blood Mononuclear Cells under Polyinosinic:Polycytidylic Acid Stimulation Document date: 2014_4_23
ID: xf4yy6i1_26
Snippet: In addition to a ranking regarding the expression stability of each gene, geNorm also calculates a normalization factor for each sample based on the most stable reference genes, which are illustrated in Figure 3 . A large variation means that the added gene has a significant effect on the normalization and should preferably be included for calculation of a reliable normalization factor. We observed the lowest variation V n /V n+1 between inclusio.....
Document: In addition to a ranking regarding the expression stability of each gene, geNorm also calculates a normalization factor for each sample based on the most stable reference genes, which are illustrated in Figure 3 . A large variation means that the added gene has a significant effect on the normalization and should preferably be included for calculation of a reliable normalization factor. We observed the lowest variation V n /V n+1 between inclusion of the fourth and fifth most stable reference gene (0.136 and 0.149 for the whole blood and PBMC).
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