Author: Peña, José; Chen-Harris, Haiyin; Allen, Jonathan E.; Hwang, Mona; Elsheikh, Maher; Mabery, Shalini; Bielefeldt-Ohmann, Helle; Zemla, Adam T.; Bowen, Richard A.; Borucki, Monica K.
Title: Sendai virus intra-host population dynamics and host immunocompetence influence viral virulence during in vivo passage Document date: 2016_4_9
ID: z7f720dj_15
Snippet: Infectivity titers for both the seed virus and material recovered from infected mice were determined by plaque titration on Vero E6 or LLC-MK2 cells using an overlay supplemented with 1 mg TCPK trypsin/ml and expressed as plaque-forming units. SeV serially diluted in sterile phosphate-buffered saline (PBS) was inoculated onto the wells and incubated at 37 C for 45 min with agitation every 15 min. Wells were overlaid with 3 ml of 2Â MEM (Life Tec.....
Document: Infectivity titers for both the seed virus and material recovered from infected mice were determined by plaque titration on Vero E6 or LLC-MK2 cells using an overlay supplemented with 1 mg TCPK trypsin/ml and expressed as plaque-forming units. SeV serially diluted in sterile phosphate-buffered saline (PBS) was inoculated onto the wells and incubated at 37 C for 45 min with agitation every 15 min. Wells were overlaid with 3 ml of 2Â MEM (Life Technologies, Gibco) with 100 IU/ml penicillin/100 mg/ml streptomycin (Gibco), 2% BSA (Gibco), 2 mg/ml of TCPK trypsin (Sigma), and 1.6% agarose. Plaques were visualized at day 3 by staining with crystal violet in 20% methanol processed as described earlier.
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