Author: Peña, José; Chen-Harris, Haiyin; Allen, Jonathan E.; Hwang, Mona; Elsheikh, Maher; Mabery, Shalini; Bielefeldt-Ohmann, Helle; Zemla, Adam T.; Bowen, Richard A.; Borucki, Monica K.
Title: Sendai virus intra-host population dynamics and host immunocompetence influence viral virulence during in vivo passage Document date: 2016_4_9
ID: z7f720dj_35
Snippet: Cytokine expression was also measured using a cytokine multiplex assay. Lung homogenates were processed according to manufacturer instructions and then analyzed using a Bio-Plex 200 system (Bio-Rad, Hercules, CA). Briefly, tissue homogenates were centrifuged for 10 min at 1,000 rpm at 4 C to remove cellular debris. Aliquots of the cleared supernatants were transferred into 96-well round bottom plates and processed for use on the Bio-Plex system. .....
Document: Cytokine expression was also measured using a cytokine multiplex assay. Lung homogenates were processed according to manufacturer instructions and then analyzed using a Bio-Plex 200 system (Bio-Rad, Hercules, CA). Briefly, tissue homogenates were centrifuged for 10 min at 1,000 rpm at 4 C to remove cellular debris. Aliquots of the cleared supernatants were transferred into 96-well round bottom plates and processed for use on the Bio-Plex system. The cytokines/chemokines were coupled to cytokine/chemokine specific multi-plex beads (R&D Systems, INC., Minneapolis, MN) . This panel measures the concentrations of cytokines and chemokines IL-1b, IL-4, IL-6, IL-10, IFN-c, CCL2 (MCP-1), CCL3 (MIP1a), CCL4 (MIP1b), CCL5 (RANTES), CXCL10 (IP-10), and tumor necrosis factor-a (TNF-a).
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