Selected article for: "Digital array and PCR Analysis software"

Author: Qin, Jian; Jones, Robert C.; Ramakrishnan, Ramesh
Title: Studying copy number variations using a nanofluidic platform
  • Document date: 2008_8_18
  • ID: prsvv6l9_15
    Snippet: There are 765 chambers in each of the 12 panels in a digital array. When single DNA molecules are randomly partitioned into these chambers, it is possible that multiple molecules could partition into the same chamber. As a result there could be more molecules in each panel than positive chambers. The true number of molecules per chamber can be estimated using a simple Poisson distribution equation as described by Sindelka et al. (15) . We have de.....
    Document: There are 765 chambers in each of the 12 panels in a digital array. When single DNA molecules are randomly partitioned into these chambers, it is possible that multiple molecules could partition into the same chamber. As a result there could be more molecules in each panel than positive chambers. The true number of molecules per chamber can be estimated using a simple Poisson distribution equation as described by Sindelka et al. (15) . We have developed a more robust computational algorithm to analyze CNV data obtained from the digital array. This algorithm has been integrated into the Digital PCR Analysis software and is detailed in (19) .

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