Targeting regenerative exosomes to myocardial infarction using cardiac homing peptide

Vandergriff, Adam; Huang, Ke; Shen, Deliang; Hu, Shiqi; Hensley, Michael Taylor; Caranasos, Thomas G.; Qian, Li; Cheng, Ke

In order to determine the cellular response to the CHP-XO therapy, immunohistochemistry was performed with several targets. To distinguish between cardiomyocytes and other cells, α-SA was used as a co-stain for immunofluorescence. Imaging of Ki67 showed that there was increased cardiomyocyte proliferation following CHP-XO treatment (Figure 4A-B) . Angiogenesis was measured by imaging of Von Willebrand factor (VwF) and showed significant increases with CHP-XO treatment (Figure 4C-D) . As the exosomes were from human origin, negative effects by an immune response were possible. To test for this, T-cells were stained for CD3 and there was no difference between the groups (Figure 4E-F) .