Author: Hao, Xin-yan; Lv, Qi; Li, Feng-di; Xu, Yan-feng; Gao, Hong
Title: The characteristics of hDPP4 transgenic mice subjected to aerosol MERS coronavirus infection via an animal nose-only exposure device Document date: 2019_10_30
ID: vkjcheaz_12
Snippet: MERS-CoV (human betacoronavirus 2cEMC/2012, complete genome GenBank: JX869059.2) was provided by the ILAS, CAMS. The virus was propagated and expanded in Vero E6 cells (American Type Culture Collection, USA) cultured and passaged at 37°C and 5% CO 2 by routine methods. Purified and concentrated progeny viruses were titrated using Vero E6 cell-based infectivity assays, and viral titers are expressed in units of 50% tissue culture infectious dose .....
Document: MERS-CoV (human betacoronavirus 2cEMC/2012, complete genome GenBank: JX869059.2) was provided by the ILAS, CAMS. The virus was propagated and expanded in Vero E6 cells (American Type Culture Collection, USA) cultured and passaged at 37°C and 5% CO 2 by routine methods. Purified and concentrated progeny viruses were titrated using Vero E6 cell-based infectivity assays, and viral titers are expressed in units of 50% tissue culture infectious dose per 100 microliters (TCID 50 /100 μL). MERS-CoV stocks at a concentration of 10 6.8 TCID 50 /100 μL were stored at −80°C.
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