Author: Nelson, John B.
Title: THE ENHANCING EFFECT OF MURINE HEPATITIS VIRUS ON THE CEREBRAL ACTIVITY OF PLEUROPNEUMONIA-LIKE ORGANISMS IN MICE Document date: 1957_8_1
ID: vhfticne_43
Snippet: The J strain of PPLO was used in all of these tests. Heart infusion agar (pH 8) was liquefied, cooled to 40°C., and diluted 1 to 5 with saline, The undiluted medium contained 1.5 per cent agar and was used as routine in the isolation of PPLO. The diluted semisolid agar was mixed with an equal volume of young PPLO cultures in 20 per cent horse serum-bouillon. Twenty-four weanlings of each mouse strain were injected intracerebrally with this inocu.....
Document: The J strain of PPLO was used in all of these tests. Heart infusion agar (pH 8) was liquefied, cooled to 40°C., and diluted 1 to 5 with saline, The undiluted medium contained 1.5 per cent agar and was used as routine in the isolation of PPLO. The diluted semisolid agar was mixed with an equal volume of young PPLO cultures in 20 per cent horse serum-bouillon. Twenty-four weanlings of each mouse strain were injected intracerebrally with this inoculum. One series of 3 cerebral passages was made in Princeton mice, starting with a brain suspension from 2 animals injected with the PPLO-agar mixture. Finally, as a control on the activity of the medium, mice of both strains were injected with a sterile mixture of agar and horse serum-bouillon.
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