Author: Li, Li; Wang, Li; Xiao, Ruijing; Zhu, Guoguo; Li, Yan; Liu, Changxuan; Yang, Ru; Tang, Zhiqing; Li, Jie; Huang, Wei; Chen, Lang; Zheng, Xiaoling; He, Yuling; Tan, Jinquan
Title: The invasion of tobacco mosaic virus RNA induces endoplasmic reticulum stress-related autophagy in HeLa cells Document date: 2011_11_21
ID: r4c1sngt_13
Snippet: HeLa cells were grown to 90% confluence and washed twice with serum-free RPMI 1640. TMV (as the positive control) or TMV-RNA was transfected by electroporation, steps being followed as per the manufacturer's instructions. TMV-RNA was transfected into cells with Lipofectamine TM 2000 (Invitrogen, 11668019), steps being followed as per the manufacturer's instructions. Cells were incubated with the mixture of Lipofectamin TM 2000-TMV-RNA at 37 • C.....
Document: HeLa cells were grown to 90% confluence and washed twice with serum-free RPMI 1640. TMV (as the positive control) or TMV-RNA was transfected by electroporation, steps being followed as per the manufacturer's instructions. TMV-RNA was transfected into cells with Lipofectamine TM 2000 (Invitrogen, 11668019), steps being followed as per the manufacturer's instructions. Cells were incubated with the mixture of Lipofectamin TM 2000-TMV-RNA at 37 • C and 5% CO 2 for 4-6 h with serum-free RPMI 1640, washed twice with serum-free RPMI 1640 and subsequently incubated at 37 • C and 5% CO 2 in excess volume of fresh serumadded RPMI 1640.
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