The invasion of tobacco mosaic virus RNA induces endoplasmic reticulum stress-related autophagy in HeLa cells

Li, Li; Wang, Li; Xiao, Ruijing; Zhu, Guoguo; Li, Yan; Liu, Changxuan; Yang, Ru; Tang, Zhiqing; Li, Jie; Huang, Wei; Chen, Lang; Zheng, Xiaoling; He, Yuling; Tan, Jinquan

In eukaryotic cells, mRNAs are partitioned between the cytosolic and ER compartments. As described in current models, this ubiquitous mRNA partitioning process is driven by a co-translational cycle of ribosome binding and release into the ER. In brief, all mRNAs are translated in the cytosol. Early in synthesis, those mRNA/RNCs (ribosome/nascent polypeptide chain complexes) engaged in the synthesis of secretory/membrane proteins are trafficked to the ER because of the presence of an SRP (signal recognition particle) [32] . Therefore we sought to determine whether TMV-RNA was driven by a co-translational cycle of ribosome binding and translation of proteins in the ER and to confirm the location of TMV-RNA.