Author: Liao, Pei-Yu; Choi, Yong Seok; Dinman, Jonathan D.; Lee, Kelvin H.
Title: The many paths to frameshifting: kinetic modelling and analysis of the effects of different elongation steps on programmed –1 ribosomal frameshifting Document date: 2010_9_7
ID: wwq0sd2r_19
Snippet: Cells with the appropriate plasmids were cultured in 1 ml Luria-Bertani (LB) medium containing 100 mg/ml ampicillin with or without 0.75 mg/ml chloramphenicol in 24-well plates for 24 h at 250 rpm and 37 C. Fluorescence was measured using a plate reader (SpectraMax M5, Molecular Devices, Sunnyvale, CA, USA). Fluorescence measurements were performed as described earlier (27) . Experimental frameshift efficiency (FS% exp ) was obtained as the ratio.....
Document: Cells with the appropriate plasmids were cultured in 1 ml Luria-Bertani (LB) medium containing 100 mg/ml ampicillin with or without 0.75 mg/ml chloramphenicol in 24-well plates for 24 h at 250 rpm and 37 C. Fluorescence was measured using a plate reader (SpectraMax M5, Molecular Devices, Sunnyvale, CA, USA). Fluorescence measurements were performed as described earlier (27) . Experimental frameshift efficiency (FS% exp ) was obtained as the ratio of green fluorescence to red fluorescence for the test strains and normalized against the fluorescence ratio of the control strain. Statistical analyses were applied to all data sets as described earlier (32) . A total of 23-46 replicates for test strains and control strains were performed to satisfy the minimum sample requirement for statistical significance.
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