Author: te Velthuis, Aartjan J.W.; van den Worm, Sjoerd H. E.; Snijder, Eric J.
Title: The SARS-coronavirus nsp7+nsp8 complex is a unique multimeric RNA polymerase capable of both de novo initiation and primer extension Document date: 2011_10_29
ID: tx0lqgff_45
Snippet: Following up on the description of an nsp8-and nsp7-containing hexadecameric ring structure (13) and the nsp8-associated polymerase activity (12), we here demonstrate that the nsp(7+8) hexadecamer is the most probable conformation of the second SARS-CoV polymerase, given the near-complete association of nsp7 and nsp8 when mixed 1:1 in solution ( Figure 2F ). Significant for our understanding of CoV RNA synthesis, we find that this complex is capa.....
Document: Following up on the description of an nsp8-and nsp7-containing hexadecameric ring structure (13) and the nsp8-associated polymerase activity (12), we here demonstrate that the nsp(7+8) hexadecamer is the most probable conformation of the second SARS-CoV polymerase, given the near-complete association of nsp7 and nsp8 when mixed 1:1 in solution ( Figure 2F ). Significant for our understanding of CoV RNA synthesis, we find that this complex is capable of binding dsRNA molecules and extending partially double-stranded RNA templates. This activity is therefore essentially comparable to the activity reported for the nsp12-RdRp (10) .
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