Title: trans-Golgi retention of a plasma membrane protein: mutations in the cytoplasmic domain of the asialoglycoprotein receptor subunit H1 result in trans-Golgi retention Document date: 1995_7_2
ID: tedj3xxz_13
Snippet: For transient expression, eDNA constructs were subcloned into the SV40-based expression vector pECE (Ellis et al., 1986) and transfected using DEAE-dextran as described by Cullen (1987) . The next day transfected cells were trypsinized and seeded onto coverslips and processed for immunofluorescence microscopy 36--40 h after transfection......
Document: For transient expression, eDNA constructs were subcloned into the SV40-based expression vector pECE (Ellis et al., 1986) and transfected using DEAE-dextran as described by Cullen (1987) . The next day transfected cells were trypsinized and seeded onto coverslips and processed for immunofluorescence microscopy 36--40 h after transfection.
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