Author: Kopertekh, Lilya; Meyer, Torsten; Freyer, Cornelia; Hust, Michael
Title: Transient plant production of Salmonella Typhimurium diagnostic antibodies Document date: 2019_2_12
ID: y47ahl3p_27
Snippet: The accumulation of recombinant scFv-TM43-E10 and scFv-Fc-TM43-E10 proteins was further monitored in a time course experiment. N. benthamiana leaves agroinfiltrated with both pLH-PVX-m and pLH-gb-PVX-m vectors carrying scFv and scFv-Fc antibody fragments were collected from three independent plants on days 2, 5, 7 and 10 and expression was assayed by immunoblot. The experiment was repeated twice. Fig. 4 shows the results of a typical experiment. .....
Document: The accumulation of recombinant scFv-TM43-E10 and scFv-Fc-TM43-E10 proteins was further monitored in a time course experiment. N. benthamiana leaves agroinfiltrated with both pLH-PVX-m and pLH-gb-PVX-m vectors carrying scFv and scFv-Fc antibody fragments were collected from three independent plants on days 2, 5, 7 and 10 and expression was assayed by immunoblot. The experiment was repeated twice. Fig. 4 shows the results of a typical experiment. This analysis indicated that the expression profile of individual proteins was similar irrespective of the expression vector. The accumulation of scFv-TM43-E10 and scFv-Fc-TM43-E10 recombinant antibodies increased steadily from day 2, peaked between days 5 and 7 and declined at 10 dpi. Temporal analysis of recombinant protein expression from pLH-PVX-m and pLH-gb-PVX-m vectors revealed that both investigated antibody fragments displayed the highest expression level at 5-7 dpi. Therefore, we selected this time point to take plant material for protein extraction.
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