Author: Kopertekh, Lilya; Meyer, Torsten; Freyer, Cornelia; Hust, Michael
Title: Transient plant production of Salmonella Typhimurium diagnostic antibodies Document date: 2019_2_12
ID: y47ahl3p_42
Snippet: In summary, we produced diagnostic scFv-TM43-E10 and scFv-Fc-TM43-E10 antibodies by means of transient expression in plants. The accumulation of recombinant proteins was enhanced by exploring the PSLV gb silencing suppressor demonstrating for the first time its applicability for biotechnological purposes. Plantderived scFv and scFv-Fc formats of TM43-E10 protein were biologically active as demonstrated by ELISA suggesting the feasibility of the N.....
Document: In summary, we produced diagnostic scFv-TM43-E10 and scFv-Fc-TM43-E10 antibodies by means of transient expression in plants. The accumulation of recombinant proteins was enhanced by exploring the PSLV gb silencing suppressor demonstrating for the first time its applicability for biotechnological purposes. Plantderived scFv and scFv-Fc formats of TM43-E10 protein were biologically active as demonstrated by ELISA suggesting the feasibility of the N. benthamiana production platform for expression of diagnostic S. Typhimurium antibodies.
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