Author: Schrom, Eva; Huber, Maja; Aneja, Manish; Dohmen, Christian; Emrich, Daniela; Geiger, Johannes; Hasenpusch, Günther; Herrmann-Janson, Annika; Kretzschmann, Verena; Mykhailyk, Olga; Pasewald, Tamara; Oak, Prajakta; Hilgendorff, Anne; Wohlleber, Dirk; Hoymann, Heinz-Gerd; Schaudien, Dirk; Plank, Christian; Rudolph, Carsten; Kubisch-Dohmen, Rebekka
Title: Translation of Angiotensin-Converting Enzyme 2 upon Liver- and Lung-Targeted Delivery of Optimized Chemically Modified mRNA Document date: 2017_4_13
ID: tulmnb32_34
Snippet: To quantify ACE2 activity, cell culture samples were lysed as for western blotting. The ACE2 activity assay was adapted from Pedersen et al. 73 30 mg total protein extract was incubated with or without DX600 (Bachem) for 20 min at RT in ACE2 reaction buffer. 10 mM synthetic substrate Mca-Y-V-A-D-A-P-K(Dnp)-OH (R&D Systems) was added to a total volume of 100 mL and incubated for at least 60 min at 37 C......
Document: To quantify ACE2 activity, cell culture samples were lysed as for western blotting. The ACE2 activity assay was adapted from Pedersen et al. 73 30 mg total protein extract was incubated with or without DX600 (Bachem) for 20 min at RT in ACE2 reaction buffer. 10 mM synthetic substrate Mca-Y-V-A-D-A-P-K(Dnp)-OH (R&D Systems) was added to a total volume of 100 mL and incubated for at least 60 min at 37 C.
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