Selected article for: "bead assay and detection limit"

Author: Mack, Ethan A.; Kallal, Lara E.; Demers, Delia A.; Biron, Christine A.
Title: Type 1 Interferon Induction of Natural Killer Cell Gamma Interferon Production for Defense during Lymphocytic Choriomeningitis Virus Infection
  • Document date: 2011_8_9
  • ID: qkwo747o_30
    Snippet: Cytokine measurements. IFN-␥ and IL-12p70 were measured in the peritoneal lavage fluid or the serum using the mouse inflammation kit cytometric bead assay (BD Biosciences). The limits of detection were 2.5 pg/ml for IFN-␥ and 10.7 pg/ml for IL-12p70. IFN-␣ and IFN-␤ were measured in the peritoneal lavage fluid or the serum using the VeriKine mouse IFN-␣ or mouse IFN-␤ ELISA kits, respectively (PBL Interferon-Source, Piscataway, NJ). T.....
    Document: Cytokine measurements. IFN-␥ and IL-12p70 were measured in the peritoneal lavage fluid or the serum using the mouse inflammation kit cytometric bead assay (BD Biosciences). The limits of detection were 2.5 pg/ml for IFN-␥ and 10.7 pg/ml for IL-12p70. IFN-␣ and IFN-␤ were measured in the peritoneal lavage fluid or the serum using the VeriKine mouse IFN-␣ or mouse IFN-␤ ELISA kits, respectively (PBL Interferon-Source, Piscataway, NJ). The limits of detection for these assays were 12.5 pg/ml for IFN-␣ and 15.6 pg/ml for IFN-␤. The IL-12p40 ELISA, with a limit of detection of 100 pg/ml, was done as previously described (8) .

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