Author: Liu, Chunxi; Liu, Tingxian; Yu, Xiaoyue; Gu, Yizhu
Title: A preliminary study on the interaction between Asn-Gly-Arg (NGR)-modified multifunctional nanoparticles and vascular epithelial cells Document date: 2017_4_1
ID: xio0g203_10
Snippet: To examine the in suit localization of CD13 on HUVEC, 1 Â 10 5 of HUVEC were seeded in 35-mm glass bottom dishes in complete medium. The culture media was removed after the cells attached to the glass, and the cells were labelled by a PEconjugated anti-CD13 antibody (1:100 dilution) for 3 h at 4 1C. Then, cells were washed twice with cold PBS before they were fixed in 4% paraformaldehyde in 0.1 mol/L PBS for 15 min. Finally, the fixed cells were.....
Document: To examine the in suit localization of CD13 on HUVEC, 1 Â 10 5 of HUVEC were seeded in 35-mm glass bottom dishes in complete medium. The culture media was removed after the cells attached to the glass, and the cells were labelled by a PEconjugated anti-CD13 antibody (1:100 dilution) for 3 h at 4 1C. Then, cells were washed twice with cold PBS before they were fixed in 4% paraformaldehyde in 0.1 mol/L PBS for 15 min. Finally, the fixed cells were examined under a Zeiss LSM700 laser confocal scanning microscope.
Search related documents:
Co phrase search for related documents- complete medium and culture medium: 1, 2, 3, 4, 5, 6, 7, 8, 9
- complete medium and laser confocal scanning microscope: 1
- complete medium and scanning microscope: 1
- confocal scanning microscope and laser confocal scanning microscope: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- confocal scanning microscope and scanning microscope: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- laser confocal scanning microscope and scanning microscope: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
Co phrase search for related documents, hyperlinks ordered by date