Title: Yeast Kex1p is a Golgi-associated membrane protein: deletions in a cytoplasmic targeting domain result in mislocalization to the vacuolar membrane Document date: 1992_12_2
ID: ucguzgdm_27
Snippet: Immunofluorescence studies were performed following the procedure of Redding et al. (1991) and used either affinity-purified anti-Kexlp antibodies or a mouse monoclonal (13D11) which is directed against the 60-kD subunit of the yeast vacuolar membrane H+-ATPase (Kane et al., 1992) . The cells were observed using an Axiophot microscope (Carl Zeiss, Inc., Thornwood, NY) (equipped for epifluorescence at excitation wavelengths appropriate for the des.....
Document: Immunofluorescence studies were performed following the procedure of Redding et al. (1991) and used either affinity-purified anti-Kexlp antibodies or a mouse monoclonal (13D11) which is directed against the 60-kD subunit of the yeast vacuolar membrane H+-ATPase (Kane et al., 1992) . The cells were observed using an Axiophot microscope (Carl Zeiss, Inc., Thornwood, NY) (equipped for epifluorescence at excitation wavelengths appropriate for the described fluors) with a 100X objective and photographed with T-Max 400 film (Eastman Kodak Co., Rochester, NY). Mitochondria and nuclei were identified by 4',6-diamidino-2-phenyl-indole staining while vacuoles were identified by differential interference contrast (Nomarski) optics.
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